Vigorous exercise, rigorous science: What scientists learned from firefighters in training

 The 11 young firefighters went through a rigorous training exercise, carrying up to 40 pounds of gear over hilly terrain during a 45-minute training exercise in the California sun. Gloves, helmets, flashlights, goggles, and more weighted them down as they sprinted through the countryside wearing fire-resistant clothing to show they were ready to serve as wildland firefighters.

When the training was over, they immediately went to the medical tent -- not to rest and recover but to give samples of their blood, saliva, and urine for analysis by a team of scientists equipped with needles, test tubes, cold packs, and the gear of their own trade.

Then, the scientists from the Department of Energy's Pacific Northwest National Laboratory (PNNL) analyzed more than 4,700 molecules -- proteins, lipids, and metabolites -- from each of the firefighters, looking to understand what happens when the body undergoes intense physical exercise. Measuring and interpreting the data from thousands of such measurements is a specialty of PNNL scientists who explore issues related to climate science and human health by analyzing millions of sensitive measurements using mass spectrometry each year.

For this study, the intent was to increase safety for first responders and others.

"Heat stress can be life threatening," said Kristin Burnum-Johnson, a corresponding author of the study. "We wanted to take an in-depth look at what's happening in the body and see if we're able to detect danger from exhaustion in its earliest stages. Perhaps we can reduce the risk of strenuous exercise for first responders, athletes, and members of the military."

As expected, the team detected hundreds of molecular changes in the firefighters. The differences before and after exercise underscored the body's efforts at tissue damage and repair, maintenance of fluid balance, efforts to keep up with increased energy and oxygen demand, and the body's attempts to repair and regenerate its proteins and other important substances.

But in the saliva, the team found some unexpected results. There was a change in the microbial mix of the mouth -- the oral microbiome -- showing that the body was increasingly on the lookout for bacterial invaders. Scientists also saw a decrease in signaling molecules important for inflammation and for fighting off viral infections.

Source: ScienceDaily